Dna And Tissue Extraction Kit Essay - 2342 Words

In this study we extensively extracted deoxyribonucleic acid (DNA) from an unidentified segment of flesh distributed amongst different lab groups. In order to do this, we dissolved the tissues and extracted only the genetic material desired using the Qiagen DNeasy Blood Tissue extraction kit. We then used forensic methods of genome identification in order to determine the genus and species of the animal These methods include gel electrophoresis, Polymerase Chain Reaction, and Cycle Sequencing Reactions. After isolating the DNA, we gathered electropherograms from the CSRs to determine the nitrogenous base frequencies of the extracted sample using a genome alignment software program called GENEIOUS. Using this aligned data, we compared the results to the online genome index GenBank using the Basic Local Alignment Search Tool (BLAST). After analyzing the results of the alignment programs and the genome index, I determined the genus and species of my group’s sample as being Crotalus durissus, a pit-viper located in Southern and Central America. This data was determined with an E-value of 2 x 10^-5 and was taken from the mitochondrial gene with a 97% accuracy match out of 187 nitrogenous base groups. Introduction: Deoxyribonucleic acid, or DNA, is the polymer of nucleic acids that make up the foundation of all life. It is the necessary code from which an organism is derived, including all amino acids, anatomic structure, and even, in some cases, behavioral patterns.Show MoreRelatedHepatocellular Carcinoma Essay869 Words   |  4 Pagescontrolling tumor invasion and angiogenesis. Although RECK mRNA is expressed in most of normal human tissues and untransformed cells, the protein level is low or undetectable in many tumor cell lines [4]. It is established that transformation of RECK inhibits tumor invasion, metastasis, and angiogenesis in animal models. Also studies show that patients with high RECK expression in tumor tissues show better survival [5]. Thus, up-regulation of RECK is considered as a expectant approach for treatingRead MoreSensitivity Of Duplex Real Time Pcr1617 Words   |  7 Pagestargeting IS1081 and IS6110 was evaluated to 19 strains of different Mycobacterial species. The real-time PCR targeting both IS1081 and IS6110 sequences showed negative result with all Mycobacterial A. Selim et el. 50 species in two concentrations of DNA from each strain, 5ng/ µl and 5pg/ µl; while strong positive result with M. bovisBCG was detected. Furthermore, ÃŽ ²-actin internal control showed positive Ct-values with all Mycobacterial species including M. bovisBCG (table 1). Table 1. MycobacteriaRead MoreRectal Cancer Case Study1065 Words   |  5 Pagesfew tumor cells (difficult to find by microscope) in fibrotic tissue; grade 4: no tumor cells, only fibrotic mass (total regression). The patient with benign rectal lesions included 23 females and 20 males; 28 of them were 65 years while 5 patients were ≠¥65 years. Clinicopathologic characteristics of rectal cancer patients are listed in Table 1. DNA extraction DNA was extracted from blood and from tissue by DNA isolation kits (QIAamp DNA Minikit, QIAGEN GmbH, Hilden, Germany) according to the instructionsRead MoreMethods And Methods Of Mouse Proteins1408 Words   |  6 Pagessequence of concern. T4, a DNA ligase will be used to anneal (recombine DNA into a double-stranded form following separation) the full-length prion sequences into an already existing pCDNA3 mammalian expression plasmid with the CMV promoter and a neomycin-resistance marker (New England Biolabs), and correct full-length prion sequences will be excised into another vector pBud egfp (Addgene) by digestion with XbaI and HindIII digestive enzymes (Promega Biolabs). Gel extraction and ligation to join fragmentedRead MoreSugarcane ( Saccharum Spp )1158 Words   |  5 Pages76-81) were grown in the field at Sugarcane Breeding Institute, Coimbatore. Crop of 10 – 12 month old were selected and disease free samples collected from the field, frozen immediately in liquid n itrogen and stored under -20oC for RNA isolation. RNA extraction and cloning of partial sequences of 4CL Homologous cloning strategy was used to clone partial sequences of 4CL. The gene sequences of 4CL genes from sorghum, maize, bamboo and rice plants were retrieved from GenBank (http://www.ncbi.nlm.nih.govRead MoreApple Scar Skin Viroid Case Study1156 Words   |  5 Pagesrapid, sensitive, and high throughput detection and can be the cornerstone of disease-risk management. Up to now, numerous diagnostic methods have been developed for the detection of virus, bacteria, fungi, and viroids by PCR, DNA-fingerprinting, aptamer biosensor, and DNA microarray, etc (1–3). Furthermore, some serological techniques have been developed for detecting functionally active bacterial cells (4). Although these molecular and serological methods are relatively efficient and accurate,Read MoreSpecific Pathogen-Free Animal Rats1525 Words   |  7 Pagesexpected outcome. Hematoxylin and Eosin staining In order to evaluate the myocardial tissue alterations in the xenograft model, rats were killed at (24) post Qingyi Decoction treating and left ventricular myocardial tissues were collected from each group and fixed in 10% formalin, processed and embedded in paraffin wax. Thin sections of 3-5 microns thickness were cut and placed on microscopic slides. The tissues were deparaffinized in xylene solution, rehydrated in downstream serial dilutions ofRead MoreThree Important Findings From This Study1414 Words   |  6 PagesCD34+ cord blood cells through viral transport (1). Firstly, lenti-JAK2 is created through restriction enzyme digestion and DNA ligation (4). The desired JAK2 DNA sequence and the bacterial plasmid are obtained and specific restriction enzymes digest both of them. JAK2 is subsequently inserted into the plasmid, sealed and combined by DNA ligase (4). The resulting plasmid DNA with the JAK2 gene-of-interest is pCDH-EF1-JAK2-T2A-GFP. It contains as well the green fluore scent protein (GFP) that helpsRead MoreDna Sequences Using Polymerase Chain Reaction1605 Words   |  7 PagesRibosomal DNA Sequences using Polymerase Chain Reaction Edwina Abou Haidar, Houssam Al Koussa, Mary AbedAlAhad. Department of Biology, Lebanese American University, Byblos, Lebanon Abstract The 16s rRNA gene sequencing is a widely common amplicon sequencing method used to identify and compare bacteria in a given sample. This method is well established and allows to study phylogeny and taxonomy of complex microbiomes. In this study, an unknown sample of extracted microbial DNA was analyzedRead MoreMolecular Genetic Experiment : Biology Lab1793 Words   |  8 PagesInduced Gene pCNT103, a Constitutive Gene GapC and a Cytokinin Induced Cig1 Gene in the DNA and RNA Extracts of Differentiated Shoot, Root, and Callus Tissue of Nicotiana tabacum Demaris Gonzalez Genetics Lab Professor Kamil Starczak March 23rd , 2017 Abstract: The experiments purpose was to understand and observe the gene expressions in the genes pCNT103, cig1 and GapC in the shoot, root and callus tissues of the tobacco plant, Nicotiana tabacum. Using various genetic laboratory research techniques